同义词 X
  • rt-pcr

    译词

  • 聚合酶链反应 (454)
  • 半定量RT-PCR (203)
  • 鉴定 (192)
  • 逆转录聚合酶链反应 (182)
  • 逆转录-聚合酶链反应 (120)
  • 免疫组织化学 (115)
  • 实时荧光定量 (83)
  • 多聚酶链反应 (67)
  • 原位杂交 (57)
  • 实时荧光定量RT-PCR (47)
  • 逆转录聚合酶链式反应 (39)
  • 原核表达 (35)
  • 逆转录多聚酶链反应 (33)
  • 半定量逆转录聚合酶链反应 (31)
  • 反转录聚合酶链反应 (30)
  • 逆转录-聚合酶链式反应 (25)
  • 反转录聚合酶链式反应 (23)
  • 逆转录-聚合酶链反应 (21)
  • 基因芯片 (21)
  • 半定量逆转录-聚合酶链反应 (21)
  • 实时定量PCR (16)
  • 反转录-聚合酶链反应 (15)
  • 反转录-聚合酶链式反应 (15)
  • 逆转录多聚酶链式反应 (11)
  • 逆转录-多聚酶链反应 (9)
  • 反转录多聚酶链反应 (8)
  • 微血 (8)
  • 巢式逆转录聚合酶链反应 (8)
  • 犬瘟热病毒 (8)
  • 反转录PCR技术 (7)
  • 半定量PCR (6)
  • 酶联免疫吸附试验 (6)
  • 亚细胞定位 (5)
  • 逆转录酶聚合酶链反应 (5)
  • 逆转录-聚合酶链反应技术 (4)
  • 结构蛋白基因 (3)
  • 逆转录聚合酶链反应法 (3)
  • 逆转录-PCR (3)
  • 逆转录—多聚酶链反应 (3)
  • 逆转录聚合酶联反应 (2)
  • 逆转录巢式PCR (2)
  • 反转录—聚合酶链反应 (2)
  • 逆转录-多聚酶联反应 (2)
  • 星形胶质细胞 (2)
  • 逆转录—聚合酶链式反应 (2)
  • 半定量反转录-聚合酶链反应 (2)
  • 逆转录-聚合酶链反应法 (2)
  • 逆转录-多聚酶链式反应 (1)
  • 反向聚合酶链反应 (1)
  • 逆转录酶-多聚酶链反应 (1)
  • 信使核糖核酸 (1)
  • 逆转录-链式聚合酶反应 (1)
  • 反转录-PCR (1)
  • 反转录-聚合酶链式反应 (1)
  • 逆转录多聚酶链反应法 (1)
  • 聚合酶链反应

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  • 1方法应用逆转录聚合酶链反应(RT-PCR)技术检测Ang-2 mRNA在65例子宫内膜异位症患者(内异症组)、40例子宫腺肌病患者(腺肌病组)的异位及在位内膜中的表达及在30例正常子宫内膜组织(对照组)中的表达。
     [Methods] Expression of Ang-2 mRNA in the eutopic and ectopic endometrium of 65 patients with endometriosis (endometriosis group) and 40 patients with adenomyosis (adenomyosis group) and the expression of Ang-2 mRNA in the normal endometrium of 30 patients (control group) without endometriosis and adenomyosis were detected by rt-pcr.
     来源:学术文献

  • 半定量RT-PCR

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  • 1对得到的AmCBL1基因在沙冬青干旱和300mM NaCl的梯度处理各0、2、4、12、24h,进行半定量RT-PCR Southern杂交,以Actin2为RNA模板等量上样的参照。
     Semi-quantity rt-pcr Southern blot was made for the total RNA of 0,2,4,12,24h drought and 300mM NaCl gradient treated respectively, using AmCBLl cDNA as the probe and Actin2 as an equal loading control.
     来源:学术文献

  • 鉴定

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  • 1连续传代5代以后,收集各W摘要代细胞至第10代,采用RT一PCR、直接荧光抗体染色和夹心ELISA技术进行鉴定,结果均为阳性。
     Cells were collected after being passaged 5 times and used to identify infection by rt-pcr, direct fluorescent antibody, sandwich ELISA.
     来源:学术文献

  • 逆转录聚合酶链反应

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  • 1在本实验中我们主要通过应用逆转录聚合酶链反应(RT—PCR)技术在基因水平检测维甲酸、氢醌等药物对B16F10小鼠黑素瘤细胞酪氨酸酶mRNA表达的调控作用,并探讨其可能的作用机制。
     In this experiment, we used reverse transcription-polymerase chain reaction (rt-pcr) to detect the regulation of tyrosinase mRNA expression of B16F10 murine melanoma cells treating by retinoic acid and hydroquinone, and get insight on the mechanisms involved.
     来源:学术文献

  • 逆转录-聚合酶链反应

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  • 1方法 应用逆转录-聚合酶链反应(RT-PCR)检测89例SLE患者和30例健康对照者外周血单一核细胞(PBMC)的IL-4R、IL-6R和IL-10R mRNA的表达水平;
     Methods rt-pcr technique was used to semiquantititively analyze the mRNA expression of IL-4R, IL-6R and IL-10R in PBMC from 89 patients with SLE and 30 healthy controls.
     来源:学术文献

  • 免疫组织化学

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  • 1实验结束后,所有动物采用免疫组织化学、Western-blot以及RT-PCR等方法检测肾上腺髓质细胞内HAP-1蛋白和mRNA水平的变化。
     The expression of HAP1 protein and the level of HAP1 mRNA in the adrenal medullas of the rats were detected by immunohistochemical and Western blot methods and rt-pcr technique,respectively.
     来源:学术文献

  • 实时荧光定量

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  • 1进一步对6个株系进行RT-PCR和实时荧光定量RT-PCR检测,结果表明外源基因能够表达,但各株系外源基因的表达水平不尽相同,表达量最高的为TL3和TL1,TL10和TL11次之、最低的为TL5和TL7。
     Real time rt-pcr method was employed to study the expression of the E2s gene, indicated that it was expressed but the expression rates varied. Among the six lines, TL3 and TL1 had the highest expression rate, TL10 and TL11 lines are lower, and TL5 and TL7 had the lowest expression rate.
     来源:学术文献

  • 多聚酶链反应

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  • 1方法 本研究应用逆转录—多聚酶链反应(RT-PCR)法和免疫组化SP法,对48例原发性肺腺癌组织中LKB1mRNA及其产物LKB1进行检测,并对临床资料与实验结果之间的关系进行了分析。
     Methods Using reverse transcription-polymerase chain reaction(rt-pcr) and SP immunohistochemistry, expression of LKB1mRNA and LKB1 was investigated in 48 primary lung adenocarcinoma tissue samples.
     来源:学术文献

  • 原位杂交

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  • 1(4) 用原位杂交和RT-PCR技术研究抑郁模型大鼠海马CA1、CA3区锥体细胞和齿状回颗粒细胞CNTF、CNTFRα mRNA表达的变化及复方刺蒺藜苷的影响。
     (4) The in-situ hybridization and reverse transcriptase PCR (rt-pcr) were employed to detect the expression of CNTF mRNA and CNTFRα mRNA in the hippocampus of rats in different groups.
     来源:学术文献

  • 实时荧光定量RT-PCR

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  • 1实时荧光定量RT-PCR检测大肠腺癌中WT1基因表达
     Real-time fluorescence quantitative rt-pcr detection of WT1 gene expression in colorectal adenocarcinoma
     来源:学术文献

  • 逆转录聚合酶链式反应

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  • 1方法 :对多株HCV核酸序列进行同源性比较设计引物 ,逆转录聚合酶链式反应 (RT -PCR)扩增 5′NCR、C、E1和E2 /NS14个编码区共 5个片段 ,分别克隆。 以连接聚合酶链反应 (PCR)将这 5个片段拼接为一连续的长 2 5 47nt的片段 ,含HCV完整的 5′NCR和全部的结构蛋白编码序列。
     METHODS and RESULTS: Primers with their sequences homologous to multiple strains of HCV had been designed, synthesized and used in rt-pcr to amplify 5 fragments from 4 regions,5'NCR,C,E1 and E2/NS1.The products had been cloned, restrictively cut aside, and overlappingly amplified to ligate a consecutive sequence of 2547 bp in length that contains the whole HCV 5'NCR and all the structural protein coding regions.
     来源:学术文献

  • 原核表达

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  • 1[方法]从人胰腺癌细胞株SW_(1990)中抽提出SHH-N末端基因,并将其插入原核表达载体pET22b中,转化E.
     [Methods] The total RNA was isolated from human pancreatic cancer strain SW_(1990) After being synthesized and amplified from the total RNA by the method of rt-pcr, the SHH-N terminal gene was cloned into pET22b vector, which was then transformed into E coli.
     来源:学术文献

  • 逆转录多聚酶链反应

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  • 1方法:在Connolly线栓法的基础上进行改良,复制大鼠MCA区2h脑缺血-再灌流2,4,8,24h动物模型,用逆转录多聚酶链反应(RT-PCR)测定缺血区皮质和皮质下组织不同时相SAMDC-mRNA的表达。
     Methods: 2 hours of reversible focal ischemia in rats were produced by improved the method of Connolly' suture occlusion of MCA and the expression of SAMDC-mRNA in the cortex and subcortex was measured by rt-pcr after 2,4,8 and 24h reperfusion following 2h ischemia respectively. The time courses of SAMDC-mRNA expression in the experimental and control group were analyzed.
     来源:学术文献

  • 半定量逆转录聚合酶链反应

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  • 1两组分别于高氧或空气暴露后1、4、7、10和14d时提取肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定Cytb mRNA表达;
     After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and cytochrome b mRNA were detected with reverse transcription polymerase chain reaction(rt-pcr).
     来源:学术文献

  • 反转录聚合酶链反应

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  • 1方法:用反转录聚合酶链反应(RT-PCR), 分别对自发性高血压大鼠(SHR)肾皮质内ACE和AT1 受体m RNA表达进行测定,观察用ACEI或AT1RA干预治疗后的变化。
     Methods:Used rt-pcr to detect the ACE and AT 1 mRNA expressions in renal cortex of spontaneous hypertensive rats(SHR) and Wistar Kyoto rats(WKY) before and after applied ACEI or AT 1RA treatment.
     来源:学术文献

  • 逆转录-聚合酶链式反应

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  • 1方法 应用靶向细胞角蛋白20(CK20)mRNA的巢式逆转录-聚合酶链式反应(nested-Reverse Transcription Polymerase Chain Reaction,nested RT-PCR)方法对40例子宫内膜癌患者的癌组织及17例患者的92枚腹膜后淋巴结进行检测,将淋巴结检测结果与常规病理学检查结果对比,并用有限系列稀释法对该扩增体系的检测敏感性进行分析。
     Methods We used the nested Reverse Transcription - Polymerase Chain Reaction (nested rt-pcr) method to detect the expression of CK20 mRNA in cancer cells from 40 patients and in 92 lymph nodes from 17 patients with endometrial carcinoma , the sensitivity of this system was investigated with limited serial dilution method.
     来源:学术文献

  • 反转录聚合酶链式反应

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  • 1这些包括定量反转录聚合酶链式反应(RT-PCR)、Northern印迹、原位杂交(参见,例如,Current Protocols inMolecular Biology(Ausubel等人,编辑,2001增刊))以及免疫测定(例如切片材料的免疫组织化学分析)、Western印迹和在完整细胞中可接近的标志物的流式细胞术分析(FACS)(参见,例如,Harlow和Lane,使用抗体:A Laboratory Manual,New York:Cold Spring Harbor Laboratory Press(1998))。
     These include quantitative reverse transcriptase polymerase chain reaction (rt-pcr), Northern blots, in situ hybridization (see, e.g., Current Protocols in Molecular Biology (Ausubel et al., eds. 2001 supplement)), and immunoassays such as immunohistochemical analysis of sectioned material, Western blotting, and for markers that are accessible in intact cells, flow cytometry analysis (FACS) (see, e.g., Harlow and Lane, Using Antibodies: A Laboratory Manual, New York: Cold Spring Harbor Laboratory Press (1998)).
     来源:专利文献   中文专利号:CN103052393A   英文专利号:US2012039955

  • 逆转录-聚合酶链反应

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  • 1其巾46例胃癌标本及其相应正常组织予逆转录-聚合酶链反应(RT-PCR)方法检测 survivin mRNA表达;
     In the present study, 46 cases of gastric cancer with corresponding normal gastric mucosa was detected survivin mRNA expression using reverse transcription-polymerase chain reaction (rt-pcr).
     来源:学术文献

  • 基因芯片

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  • 1通过基因芯片检测60μmol/L DADS作用于HL-60细胞4h后凋亡相关基因的表达谱,采用RT-PCR技术验证上调基因Fas-L和下调基因Bag-1。
     The expression profile of apoptosis-associated genes in HL-60 cells, with or without 4-hour treatment of DADS (60 μmol/L), was identified by gene array. The up-regulated Fas-L gene and down-regulated Bag-1 gene were confirmed by reverse transcription-polymerase chain reaction (rt-pcr).
     来源:学术文献

  • 半定量逆转录-聚合酶链反应

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  • 1半定量逆转录-聚合酶链反应(RT-PCR)技术检测U251细胞感染HCMV和/或全反式维甲酸(ATRA)处理前后HOXB1,HOXB2,HOXB3,HOXB4和HOXB9基因的相对表达水平。
     The expressions of HOXB1, HOXB2, HOXB3, HOXB4 and HOXB9 genes in U251 cells infected with HCMV and/or treated with ATRA were detected by semi-quantitative rt-pcr.
     来源:学术文献

  • 实时定量PCR

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  • 1结果:实时定量PCR方法的最大灵敏度可达到10拷贝/μl,但重复性欠佳;
     Results The sensitivity of real-time rt-pcr was 10copies/μl.
     来源:学术文献

  • 反转录-聚合酶链反应

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  • 1②分别于诱导分化的第2,4,6,8天采集细胞,采用反转录-聚合酶链反应检测脂肪细胞抵抗素mRNA的表达,以免疫印迹分析方法检测脂肪细胞抵抗素蛋白的表达。
     ②The adipocytes were collected on the 2nd, 4th, 6th and 8th days. The expression of 3T3-L1 adipocytes resistin mRNA was detected by reverse transcription-polymerase chain reaction (rt-pcr) and expression of resistin protein was detected by Western blotting.
     来源:学术文献

  • 反转录-聚合酶链式反应

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  • 1为了研究EGF在猪雌性生殖器官中的作用,我们选择了反转录-聚合酶链式反应(RT-PCR)的方法检测了EGF的mRNA在猪卵巢、输卵管和子宫中的表达情况。
     To study the role of EGF mRNA hi female reproductive organ of pig, reverse transcription-polymerase chain reaction (rt-pcr) was used to examine the expression of EGF mRNA in the ovaryoviduct and uterus.
     来源:学术文献

  • 逆转录多聚酶链式反应

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  • 1方法:采用分别以绿色(PML)和橘红色(RARα)两种荧光素直接标记的PML和RARα基因探针,对55例APL患者的骨髓涂片或骨髓细胞悬液制片进行双色荧光原位杂交(D-FISH),并和常规核型分析(CC)和逆转录多聚酶链式反应(RT-PCR)结果比较。
     Methods:Sequence special probes for PML and RAR a genes directly labeled with SpectrumGreen and Spectrum Orange respectively and D-FISH were used to detect PML/RARa rearrangement in 55 APL patients and the results were compared with that by conventional cytogenetics (CC) and reverse transcriptase polymerase chain reaction (rt-pcr).
     来源:学术文献

  • 逆转录-多聚酶链反应

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  • 1逆转录-多聚酶链反应(RT-PCR)检测BMSCs/tk对tk基因的转录。
     rt-pcr was performed to examine the transduct of tk gene after it was transduced into BMSCs.
     来源:学术文献

  • 反转录多聚酶链反应

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  • 1标本共 16份 ,提取 m RNA后经反转录多聚酶链反应 (RT- PCR) ,PCR产物进一步经基因扫描分析 Vβ2 4个亚家族基因的互补决定区 3(CDR3)以确定 T细胞克隆性。
     The complementarity determine region 3 (CDR3) of 24 TCRVβ subfamilies genes were amplified by rt-pcr, the PCR products were further analyzed by Genescan to identify the clonality of the T cells.
     来源:学术文献

  • 微血

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  • 1通过建立实时荧光定量RT-PCR方法,检测人微血管内皮细胞t-PA mRNA的表达。
     A realtime rt-pcr was constructed for detecting t-PA mRNA expression from Human microvascular endothelial cell (HMEC-1).
     来源:学术文献

  • 巢式逆转录聚合酶链反应

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  • 1方法以巢式逆转录聚合酶链反应(RT-PCR)技术检测58例贲门癌患者术前、术中、术后外周血CK20mRNA的表达情况。
     Methods CK20mRNA in peripheral blood of 58 patients with cardial carcinoma was detected before. during and after resection by using nested rt-pcr technique.
     来源:学术文献

  • 犬瘟热病毒

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  • 1RT-PCR检测贵州犬瘟热病毒
     rt-pcr detection of canine distemper virus from dogs in Guizhou area
     来源:学术文献

  • 反转录PCR技术

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  • 1采用免疫细胞化学染色技术测定NF-κB、TNF-α表达,采用酶免疫试验测定TNF-α含量,采用反转录PCR技术测定TNF-αmRNA表达。
     (3) TNF-αconcentration (enzyme immunometric assay EIA) and TNF-αmRNA expression (rt-pcr) .
     来源:学术文献

  • 半定量PCR

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  • 12.以持家基因β-Actin作为内参照,对靶基因(NCED)进行半定量PCR扩增,发现低温(4℃)胁迫处理后,NCED基因的表达量逐步增加;
     2. Consructed the optimized semi-quantitative rt-pcr to detect NCED gene expression difference after different time of exposed to cold, using P-Actin as inner control.
     来源:学术文献

  • 酶联免疫吸附试验

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  • 1目的探讨人疱疹病毒6型(HHV-6)感染的免疫发病机理。 方法用逆转录-聚合酶链反应(RT-PCR)和双抗体夹心酶联免疫吸附试验(ELISA)检测白细胞介素-10(IL-10)等细胞因子的产生。
     In this report,interleukin 10(IL-10)and other cytokines were evaluated through reverse transcription-polymerase chain raction (rt-pcr)and sandwich ELISA to explore possible immune pathogenesis of human herpesvirus 6 (HHV-6) infection.
     来源:学术文献

  • 亚细胞定位

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  • 1运用荧光定量PCR(Real time RT-PCR)、免疫印迹和免疫细胞化学法分析SSeCKS的表达变化和亚细胞定位
     The expression of SSeCKS was detected by Real time rt-pcr and Western blotting,while immunocytochemistry was used to investigate the subcellular localization of it.
     来源:学术文献

  • 逆转录酶聚合酶链反应

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  • 1通过现代分子生物学方法,例如实时逆转录酶聚合酶链反应(实时RT-PCR)或基因表达芯片分析对细胞内转录模板(mRNA模板)进行的定量分析能够鉴定(例如)不正确表达的基因,以便检测,例如,代谢疾病、感染或癌症的存在。
     Quantitative analysis of transcription templates (mRNA templates) in cells by means of modern molecular biological methods, such as real time reverse transcriptase polymerase chain reaction (real time rt-pcr) or gene expression chip analysis enables, for example the identification of incorrectly expressed genes, so that, for example, metabolic disorders, infections or the presence of cancer can be detected.
     来源:专利文献   中文专利号:CN101611302A   英文专利号:US2010317051

  • 逆转录-聚合酶链反应技术

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  • 1方法采用半定量逆转录-聚合酶链反应技术、原位杂交技术、免疫组化技术等,观察家兔心肌缺血/再灌注模型缺血交界区caspase-3 mRNA、p53 mRNA、bcl-2 mRNA动态表达的内在联系。
     Methods The relation of Caspase-3 mRNA,p53 mRNA,and the bcl-2 mRNA expressions at the boundary area of the myocardial ischemia reperfusion was detected in the myocardial ischemia/ reperfusion model of rabbit by semi-rt-pcr and in situ hybridization and immunohistochemistry.
     来源:学术文献

  • 结构蛋白基因

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  • 1通过RT-PCR法获得阿克苏(Akesu/O/58)FMDV结构蛋白基因vp1,将该基因克隆到pGEM-T Easy载体进行核苷酸序列测定。
     The structural gene vp1 was obtained by reverse transcriptase-polymerase chain reaction(rt-pcr) from RNA of foot-and-mouth disease virus strain Akesu/58 Serotype O.
     来源:学术文献

  • 逆转录聚合酶链反应法

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    变形:   english【复】

  • 1分别通过逆转录聚合酶链反应法(RT-PCR)和免疫组织化学方法检测Slit2基因的表达水平及部位。
     The expressions of Slit2 were tested by reverse transcription polymerase chain reaction (rt-pcr) and immunohistochemical measurement.
     来源:学术文献

  • 逆转录-PCR

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  • 1方法应用逆转录-PCR(RT-PCR)法检测41例SLE患者及32例年龄、性别与之相匹配的正常人PBMC中bcl-2mRNA与bax mRNA的表达,并用免疫印迹法检测SLE患者组自身抗体谱。
     Methods The levels of the expression of mRNA of bcl-2 and bax of 41 SLE patients and control group of 32 healthy persons which age and sex-matched were detected by rt-pcr; While anti-ENA autoantibodies of SLE patients were detected with Western-blot.
     来源:学术文献

  • 逆转录—多聚酶链反应

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  • 1逆转录—多聚酶链反应(RT-PCR)检测20例新鲜喉癌及相应癌旁组织FHIT和C-myc mRNA表达情况。
     FHIT and C-myc mRNA expression were detected with rt-pcr in 20 cases of fresh laryngeal carcinom samples and its matched contrast groups.
     来源:学术文献

  • 逆转录聚合酶联反应

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  • 1用免疫组化法和逆转录聚合酶联反应法(RT-PCR),检测肺组织中COX-2和PDGF-A及PDGF-B的表达情况。
     The expressions of COX-2 and PDGF-A and PDGF-B in the lung tissue were detected by immunhistochemistry and rt-pcr.
     来源:学术文献

  • 逆转录巢式PCR

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  • 1人肝癌组织GGT异常表达与逆转录巢式PCR分析
     Abnormal Expression and rt-pcr Analysis of γ-Glutamyl Transpeptidase Genes in the Tissues of Human Hepatocelullar Carcinoma
     来源:学术文献

  • 反转录—聚合酶链反应

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  • 1根据Genebank已发表的PRV VP7基因cDNA序列,利用Oligo4.1软件设计并合成一对引物,通过反转录—聚合酶链反应(RT—PCR)扩增出长约1.0kb的基因片段,命名为VP7。
     A Pair of prirners was designed to amplify VP7 gene by rt-pcraccording to the published Sequence of PRVs VP7 gene with Oligo4. l software.
     来源:学术文献

  • 逆转录-多聚酶联反应

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  • 1TUNEL法检测细胞凋亡; 逆转录-多聚酶联反应(RT-PCR)检测模型大鼠脑神经细胞bcl-2基因的表达情况。
     TUNEL methods was used on the cell apoptosis, rt-pcr method was used on the expression of bcl-2 in the nerve cell of hypothyroidism rats.
     来源:学术文献

  • 星形胶质细胞

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  • 1我们用RT-PCR和Western blot方法首次证明RGS2、RGS5存在于星形胶质细胞中。
     Using rt-pcrand western blot analysis, we first found RGS2 and 5 were expressed in striatalastrocytes.
     来源:学术文献

  • 逆转录—聚合酶链式反应

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  • 1采用逆转录—聚合酶链式反应(RT-PCR)、放射免疫测定(RIA)、原位杂交(ISH)和免疫组织化学(IHC)等方法,观察应激致高血压过程中大鼠延髓ppADM及其特异性受体组件—降钙素受体样受体(CRLR)、受体活性修饰蛋白2和3(RAMP2和RAMP3)的表达以及ADM含量变化;
     To detect the dynamic changes of preproadrenomedullin (ppADM) gene encoding ADM and its specific receptor components, calcitonin receptor-like receptor (CRLR) and the receptor activity modifying protein 2 and 3 (RAMP2 and RAMP3) in the medulla oblongata within consecutive stress for 15 days, we used reverse transcription-polymerase chain reaction (rt-pcr), Radioimmunoassay(RIA), in situ hybidization (ISH) and immunohistochemistry methods.
     来源:学术文献

  • 半定量反转录-聚合酶链反应

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  • 1半定量反转录-聚合酶链反应(RT-PCR)显示IL-2及IFN-γmRNA的表达。
     The IL-2and IFN-γmRNA expressions were determined by rt-pcr.
     来源:学术文献

  • 逆转录-聚合酶链反应法

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    变形:   english【复】

  • 1逆转录-聚合酶链反应法(RT-PCR)及免疫组化方法检测TGF-β1mRNA和蛋白表达情况。
     The mRNA and protein expression of TGF-β1 was examined by reverse transcription-polymerase chain reaction(rt-pcr) and immunohistochemistry.
     来源:学术文献

  • 逆转录-多聚酶链式反应

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  • 1报道逆转录-多聚酶链式反应(RT-PCR)联合一步程序,扩增大于1kb的拟南芥菜G蛋白α亚基基因的开放阅读框。
     It is reported that a one-step reverse transcription and polymerase chain reaction (rt-pcr)procedure for amplification of open reading frame of G-protein αsubunit gene from Arabidopsis thaliana which is larger than 1 kb.
     来源:学术文献

  • 反向聚合酶链反应

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  • 1方法采用酶联免疫吸附试验(ELISA)和反向聚合酶链反应(RT-PCR)技术分别对SLE患者和并发LN的SLE患者血浆中可溶性EPCR(sEPCR)水平和外周血循环系统内皮细胞蛋白C受体mRNA的表达进行检测。
     Methods The soluble endothelial protein C receptor (sEPCR) in plasma and EPCR mRNA in peripheral blood circulating endothelial protein C receptor(EPCR) were detected by enzyme linked Immunosorbent assay(ELISA) and reverse transcription-polymerase chain reaction(rt-pcr) in patients with systemic lupus erythematosus(SLE),LN and normal control,respectively.
     来源:学术文献

  • 逆转录酶-多聚酶链反应

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  • 1方法 应用逆转录酶-多聚酶链反应(RT-PCR)方法,检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达,并与相应癌旁组织进行对照分析。
     Methods rt-pcr was applied to study expression of MRP gene in tumor tissues from 83 cases of esophageal and cardiac carcinoma, and 45 lymph nodes from 28 patients.
     来源:学术文献

  • 信使核糖核酸

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  • 1合适的信使核糖核酸的检测方法包括Northern印迹(Northern blotting),反转录聚合酶链式反应(RT-PCR)。
     Suitable mRNA detection methods include Northern blotting and reverse transcriptase polymerase chain reaction (rt-pcr).
     来源:专利文献   中文专利号:CN102317787A   英文专利号:US2010129853

  • 逆转录-链式聚合酶反应

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  • 1方法随机抽取上海交通大学医学院附属新华医院1999年9月至2003年5月收治的93例女性乳腺癌病人的临床资料。 采用逆转录-链式聚合酶反应(reverse transcription-polymerase chain reaction,RT-PCR)检测标本BCSG1 mRNA表达,统计分析BC-SG1基因表达与乳腺癌各项临床病理参数的相关性;
     Methods The expression of BCSG1 was detected by reverse transcription-polymerase chain reaction (rt-pcr) in surgical specimens from 93 cases of breast cancer patients selected randomly between September 1999 and May 2003 at Xinhua Hospital,Shanghai Jiaotong University School of Medicine. The correlation between BCSG1 gene expression and clinical parameters of breast cancer was analyzed.
     来源:学术文献

  • 反转录-PCR

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  • 1我们用反转录-PCR方法从人白细胞总RNAs扩增出人α防御素3前体的编码序列,克隆到pDEST17表达载体,表达不同结构的人α防御素3蛋白。
     cDNA of human pre-pro-HNP-3 was rt-pcr amplified from total RNAs of human white blood cells and cloned into pDEST17 expression vector.
     来源:学术文献

  • 反转录-聚合酶链式反应

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  • 1应用反转录-聚合酶链式反应方法,测定大鼠ET-A型受体(endothelinareceptor,ETA-R)mRNA在体内的分布,证明ETA-RmRNA不仅存在于血管平滑肌细胞中,亦存在于内皮细胞中;
     With reverse transcription-polymerase chain reaction(rt-pcr),expression of endothelin A receptor (ETA-R ) mRNA in rats has been detected.
     来源:学术文献

  • 逆转录多聚酶链反应法

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    变形:   english【复】

  • 1本实验采用免疫组织化学法和定量逆转录多聚酶链反应法(RT-PCR)检测Lewis 肺癌化疗小鼠肿瘤转移抑制基因(nm23-M1)的蛋白及 mRNA 表达,探讨天灸对肺癌化疗小鼠的增效机理。
     The experiment was made to discuss the mechanism of increasing curative effectof Lewis lung carcinoma chemotherapy-therapy mice which were given tianjiutherapy through measuring protein and mRNA expression of nm23-M1 the micethrough immunohistochemical method and real-time fluorescence quantitativeReverse Transcription-Polymerase Chain Reaction (rt-pcr).
     来源:学术文献

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